Respuesta frente a Phytophthora palmivora (Butl.) Butl. en accesiones de cacao en la Estación Experimental Agro-Forestal Baracoa, Cuba1
Article Sidebar
Main Article Content
Abstract
With the objective of determining the answer forehead of Phytophthora palmivora of 30 accession of cocoa of the germplasm bench of the Estación Experimental Agro-Forestal of Baracoa the following work was developed, in the period understood among April to December of 2018, on a totally randomized design with 10 repetitions. Initially corn affected by the disease in the field were recollected, which were transferred to the laboratory and small portions of them were sowed in petri badges with cultivation medium Agar V8, traditionally used for the growth of this pathogen, at 20 days later got ready the inoculum and 10 healthy fruits of each accession were infested under field conditions (stuck to the plants), to which were placed two disks of filter paper, submerged in the previously upset suspension of spores, in opposed sides to the “equator” of the fruit (up and under the center of the fruit), and these were protected by a period of 10 days, inside a bag of nylon semi transparent, with a cotton humidified in 50 ml of distilled water. The qualitative results showed that 20 % of the accession was resistant, 27 % moderately resistant, 17 % moderately susceptible and 36 % susceptible to Phytophthora palmivora (Butl) Butl. The severity of the disease was very high, being destroyed the inoculated fruits of 91 % of the susceptible accessions completely.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Those authors who have publications with this journal accept the following terms of the License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0):
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
The journal is not responsible for the opinions and concepts expressed in the works, they are the sole responsibility of the authors. The Editor, with the assistance of the Editorial Committee, reserves the right to suggest or request advisable or necessary modifications. They are accepted to publish original scientific papers, research results of interest that have not been published or sent to another journal for the same purpose.
The mention of trademarks of equipment, instruments or specific materials is for identification purposes, and there is no promotional commitment in relation to them, neither by the authors nor by the publisher.
How to Cite
References
Hubeaux, D.: Caracterisation du Phytophthora, agent de la pourriture brune de la cabosse, à Cuba. B. Sc. Louvain: Université Catholique de Louvain, Belgique. 90 pp., 2010.
Efombagn, M. I. B.; Bieysse, D.; Nyassé, S. and A. B. Eskes: “Selection for resistance to Phytophthora pod rot of cocoa (Theobroma cacao L.) in Cameroon: Repeatability and reliability of screening tests and field observations.” Crop Protection, 30: 105-110, 2011.
Hernández-Rodríguez, Annia; Yeter Ruíz-Beltrán; Yanelis Acebo-Guerrero; Yulien Miguélez-Sierra y Mayra Heydrich-Pérez: Antagonistas microbianos para el manejo de la pudrición negra del fruto en Theobroma cacao L. Estado actual y perspectivas de uso en Cuba. Rev. Protección Vegetal, 29 (1):11-19, 2014.
Martínez, E. y L. Pérez: Incidencia de enfermedades fúngicas en plantaciones de cacao de las provincias orientales de Cuba. Rev. Protección Vegetal, 30 (2): 87-96, 2015.
Matos, Y.; Clapé, P. y A. Nariño: Resistencia a Phytophthora palmivora de 48 accesiones de cacao del banco de germoplasma de la Estación Experimental Agro-Forestal Baracoa, Cuba. Café Cacao, 15 (2): 28-32, 2016.
Matos, Y.; Belkys Peteira; Matos, G. A.; Decock, C.; Hubeaux, D.; Lambertt, W.; Yanelis Acebo; Ochoa, P. y P. Clapé: Prueba de apareamiento en 90 aislamientos de Phytophthora, provenientes de frutos enfermos de cacao (Theobroma cacao Lin.) en el municipio de Baracoa, provincia Guantánamo, Cuba. Revista de Protección Vegetal, 26 (3): 198-199, 2011.
Matos, Y.; Bidot, I.; Decock, C. y P. Clapé: Resistencia a Phytophthora palmivora (Butl) Butl en 25 accesiones de cacao en Cuba. Café Cacao, 12 (2): 35-38, 2013.
Melnick, R. L.; Suárez, C.; Bailey, B. A. and P. A Backman: Isolation of endophytic endospore - forming bacteria from Theobroma cacao as potential biological control agents of cacao diseases. Biological Control, 57:236245, 2011.
Motamayor, J. C.; Lachenaud, P.; da Silva e Mota, J. W.; Loor, R. and D. N. Kuhn: Geographic and genetic population differentiation of the Amazonian chocolate tree (Theobroma cacao L). PLoS ONE. 2008. 3:e3311, 10.1371/journal.pone.0003311.
Phillips, W. y J. Galindo: Método de inoculación y Evaluación de la resistencia a Phytophthora palmivora en frutos de cacao (Theobroma cacao). Turrialba, 39(4): 488-496, 1989.
Ploetz, R. C.: Cacao diseases: Important threats to chocolate production worldwide. Phytopathology, 97:16341639, 2007.
Ramírez Gil, J. G.: Pérdidas económicas asociadas a la pudrición de la mazorca del cacao causada por Phytophthora spp.,y Moniliophthora roreri (Cif y Par) Evans et al., en la hacienda Theobroma, Colombia. Rev. Protección Vegetal, 31 (1): 42- 49, 2016.
Tchameni, S. N.; Ngonkeu, M. E. L.; Begoude, B. A. D.; Wakam, N. L.; Fokoma, R. and A. D. Owona et al.: Effect of Trichoderma asperellum and arbuscular mycorrhizal fungi on cacao growth and resistance against black pod disease. Crop Protection, 30: 1321-1327, 2011.